European Journal of Obstetrics & Gynecology and Reproductive Biology
Volume 136, Issue 2 , Pages 155-159, February 2008

Enzyme linked immunosorbent assay (ELISA) as screening method for anti-paternal allo-antibodies in patients with recurrent pregnancy loss (RPL)

  • Nina Rogenhofer

      Affiliations

    • Endocrinology and Reproductive Medicine, Department of Obstetrics and Gynecology, Ludwig-Maximilians-University, München Großhadern, Marchioninistrasse 15, D-81377 München, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +49 8970950; fax: +498970953844.
    • ,
  • Bettina Toth

      Affiliations

    • Endocrinology and Reproductive Medicine, Department of Obstetrics and Gynecology, Ludwig-Maximilians-University, München Großhadern, Marchioninistrasse 15, D-81377 München, Germany
    • ,
  • Stephan Kiessig

      Affiliations

    • German Association for Humanplasma, Ludwigshafen, Germany
    • ,
  • Peter Hellstern

      Affiliations

    • Institute of Hemostaseology and Transfusion Medicine, Klinikum Ludwigshafen, Germany
    • ,
  • Uwe Taborski

      Affiliations

    • German Association for Humanplasma, Ludwigshafen, Germany
    • ,
  • Siegfried Scholz

      Affiliations

    • Laboratory for Immungenetics, Childrens Hospital, Ludwig-Maximilians-University, Munich, Germany
    • ,
  • Christian J. Thaler

      Affiliations

    • Endocrinology and Reproductive Medicine, Department of Obstetrics and Gynecology, Ludwig-Maximilians-University, München Großhadern, Marchioninistrasse 15, D-81377 München, Germany
    • Tel.: +49 8970950; fax: +49 8970954588.

Received 10 February 2006; received in revised form 18 September 2006; accepted 5 March 2007. published online 11 April 2007.

Abstract 

Objectives

Non-HLA-specific anti-paternal antibodies (APA) have been associated with immune responses against HLA-negative trophoblast. As screening for APA by using the flow cytometric cross match (FCXM) is complicated, we evaluated the One Lambda Antigen Tray Test (OLATT) an easy screening method for antibodies in organ transplant recipients.

Study design

We randomly selected 92 patients of our recurrent pregnancy loss (RPL)-clinic merely on the basis of having had at least two consecutive miscarriages at <20 weeks representing positive and negative FCXM results. Stored sera were thawed and tested by OLATT. Concordance (Kappa statistic) and conformance (Chi-square test to McNemar) of FCXM and OLATT results were analysed.

Results

Of 48 FCXM-positive patients, 38 (79.2%) were positive and 10 (20.8%) negative by OLATT. Out of 44 FCXM negative patients, 37 (84.1%) were negative and 7 (15.9%) positive by OLATT. This resulted in a positive prediction value of 84.4%, a negative prediction value of 78.7% and a highly significant concordance (κ=0.631 (p<0.0001; 81.5% versus 50%)) and conformance (p=0.23) between FCXM and OLATT in RPL patients.

Conclusion

Using the OLATT could substitute the FCXM in screening RPL patients for APA and this might help to more closely study the role of APA in human gestation.

Keywords: Recurrent pregnancy loss, ELISA, One Lambda Test, FCXM, Cytometric crossmatch, Miscarriage, Abortion, Anti-paternal antibodies

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PII: S0301-2115(07)00128-5

doi:10.1016/j.ejogrb.2007.03.002

European Journal of Obstetrics & Gynecology and Reproductive Biology
Volume 136, Issue 2 , Pages 155-159, February 2008

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