European Journal of Obstetrics & Gynecology and Reproductive Biology
Volume 143, Issue 2 , Pages 107-111 , April 2009

Methotrexate induces apoptosis of human choriocarcinoma cell line JAR via a mitochondrial pathway

  • Ya-Xia Chen

      Affiliations

    • Women's Hospital, School of Medicine, Zhejiang University, No. 2 Xueshi Road, Hangzhou 310006, China
    • Tel.: +86 571 87061501x2151; fax: +86 571 87036290.
  • ,
  • Wei-Guo Lv

      Affiliations

    • Women's Hospital, School of Medicine, Zhejiang University, No. 2 Xueshi Road, Hangzhou 310006, China
  • ,
  • Huai-Zeng Chen

      Affiliations

    • Women's Reproductive Health Laboratory of Zhejiang Province, No. 2 Xueshi Road, Hangzhou 310006, China
  • ,
  • Feng Ye

      Affiliations

    • Women's Reproductive Health Laboratory of Zhejiang Province, No. 2 Xueshi Road, Hangzhou 310006, China
  • ,
  • Xing Xie

      Affiliations

    • Women's Hospital, School of Medicine, Zhejiang University, No. 2 Xueshi Road, Hangzhou 310006, China
    • Corresponding Author InformationCorresponding author. Tel.: +86 571 87061501x1001; fax: +86 571 87036290.

Received 4 May 2008 ,Revised 7 December 2008 ,Accepted 28 December 2008.

References 

  1. Benedet JL, Bender H, Jones H, Ngan HY, Pecorelli S. FIGO staging classifications and clinical practice guidelines in the management of gynecologic cancers. FIGO Committee on gynecologic oncology. Int J Gynaecol Obstet. 2000;70:209–262
  2. Schmitz I, Kirchhoff S, Krammer PH. Regulation of death receptor-mediated apoptosis pathways. Int J Biochem Cell Biol. 2000;32:1123–1136
  3. Bernardi P, Krauskopf A, Basso E, et al. The mitochondrial permeability transition from in vitro artifact to disease target. FEBS J. 2006;273:2077–2099
  4. Forte M, Bernardi P. The permeability transition and BCL-2 family proteins in apoptosis: co-conspirators or independent agents?. Cell Death Differ. 2006;13:1287–1290
  5. Danial NN, Korsmeyer SJ. Cell death: critical control points. Cell. 2004;116:205–219
  6. Fadeel B, Orrenius S. Apoptosis: a basic biological phenomenon with wide-ranging implications in human disease. J Intern Med. 2005;258:479–551
  7. Adrain C, Martin SJ. The mitochondrial apoptosome: a killer unleashed by the cytochrome seas. Trends Biochem Sci. 2001;26:390–397
  8. Antonsson B. Mitochondria and the Bcl-2 family proteins in apoptosis signaling pathways. Mol Cell Biochem. 2004;256–257:141–155
  9. Sharpe JC, Arnoult D, Youle RJ. Control of mitochondrial permeability by Bcl-2 family members. Biochim Biophys Acta. 2004;1644:107–113
  10. Nisolle M, Casanas-Roux F, Donnez J. Immunohistochemical analysis of proliferative activity and steroid receptor expression in peritoneal and ovarian endometriosis. Fertil Steril. 1997;68:912–919
  11. Jing XB, Cai XB, Hu H, Chen SZ, Chen BM, Cai JY. Reactive oxygen species and mitochondrial membrane potential are modulated during CDDP-induced apoptosis in EC-109 cells. Biochem Cell Biol. 2007;85:265–271
  12. Kim R. Recent advances in understanding the cell death pathways activated by anticancer therapy. Cancer. 2005;103:1551–1560
  13. Kim R, Emi M, Tanabe K. Role of mitochondria as the gardens of cell death. Cancer Chemother Pharmacol. 2006;57:545–553
  14. Belmokhtar CA, Hillion J, Dudognon C, et al. Aopotosome-independent pathway for apoptosis. Biochemical analysis of APAF-1 defects and biological outcomes. J Biol Chem. 2003;278:29571–29580
  15. Vaux DL, Korsmeyer SJ. Cell death in development. Cell. 1999;96:245–254
  16. Sun X-M, MacFarlane M, Zhuang J, Wolf BB, Green DR, Cohen GM. Distinct caspase cascades are initiated in receptor-mediated and chemical-induced apoptosis. J Biol Chem. 1999;274:5053–5060
  17. Schimmer AD, Hedley DW, Penn LZ, Minden MD. Receptor- and mitochondrial-mediated apoptosis in acute leukemia: a translational view. Blood. 2001;98:3541–3553
  18. Duan X, Zhou Z, Jia SF, Colvin M, Lafleur EA, Kleinerman ES. Interleukin-12 enhances the sensitivity of human osteosarcoma cells to 4-hydroperoxycyclophosphamide by a mechanism involving the Fas/Fas-ligand pathway. Clin Cancer Res. 2004;10:777–783
  19. Ofir R, Seidman R, Rabinski T, et al. Taxol-induced apoptosis in human SKOV3 ovarian and MCF7 breast carcinoma cells is caspase-3 and caspase-9 independent. Cell Death Differ. 2002;9:636–642
  20. Tafani M, Cohn JA, Karpinich NO, Rothman RJ, Russo MA, Farber JL. Regulation of intracellular pH mediates Bax activation in HeLa cells treated with staurosporine or tumor necrosis factor-alpha. J Biol Chem. 2002;277:569–576
  21. Campello S, De Marchi U, Szabo I, Tombola F, Martinou JC, Zoratti M. The properties of the mitochondrial megachannel in mitoplasts from human colon carcinoma cells are not influenced by Bax. FEBS Lett. 2005;579:3695–3700

PII: S0301-2115(09)00008-6

doi: 10.1016/j.ejogrb.2008.12.009

European Journal of Obstetrics & Gynecology and Reproductive Biology
Volume 143, Issue 2 , Pages 107-111 , April 2009