Inflammatory cytokine mRNA detection by real time PCR in chorioamniotic membranes from pregnant women with preterm premature rupture of membranes

Polettini, J.; Peraçoli, J.C.; Candeias, J.M.G.; Araújo Júnior, J.P.; Silva, M.G.

doi:10.1016/j.ejogrb.2009.01.008

« Previous Next »European Journal of Obstetrics & Gynecology and Reproductive Biology
Volume 144, Issue 1 , Pages 27-31, May 2009

Inflammatory cytokine mRNA detection by real time PCR in chorioamniotic membranes from pregnant women with preterm premature rupture of membranes

J. Polettini
- Department of Pathology, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil
J.C. Peraçoli
- Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil
J.M.G. Candeias
- Department of Microbiology and Immunology, Institute of Biosciences, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil
J.P. Araújo Júnior
- Department of Microbiology and Immunology, Institute of Biosciences, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil
M.G. Silva
- Department of Pathology, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil
- Corresponding author at: Department of Pathology, Botucatu Medical School, São Paulo State University, Distrito de Rubião Júnior, CEP: 18618-970, Botucatu, São Paulo, Brazil. Tel.: +55 14 3811 6238; fax: +55 14 3815 2348.

Received 3 July 2008; received in revised form 9 December 2008; accepted 19 January 2009. published online 11 February 2009.

Abstract

Objective

To quantify the expression of interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor alpha (TNF-α) in chorioamniotic membranes of PPROM pregnant women with chorioamnionits.

Study design

The study included 25 PPROM women in labor, 15 PPROM without labor, and 25 pregnant women in preterm labor (PTL). Chorioamniotic membranes were collected for histopathological analyses and cytokine mRNA expression quantification by real time PCR. Comparisons were performed using the Mann–Whitney, Kruskal–Wallis, Fisher's exact test or z test with significance set at p<0.05. The software employed was the SigmaStat version 3.1.

Results

During the study PPROM incidence was 4.6% and chorioamnionits was present in 75% of the samples. IL-1β, IL-6, and IL-8 mRNA expression did not statistically differ among study groups. TNF-α mRNA expression was statistically higher in PTL. No difference in the mRNA concentration of the cytokines studied in the presence of chorioamnionitis was observed.

Conclusion

Chorioamniotic membranes are sources of IL-1β, IL-6, IL-8, and TNF-α and their mRNA concentrations in PPROM are not related to the presence of chorioamnionitis.

Keywords: Preterm premature rupture of membranes, Chorioamnionitis, Cytokines, Real time PCR