Abstract
Objective: The contribution of genetic factors to preeclampsia has been well documented. However,
there has not been any study done on the association between preeclampsia and the
angiotensinogen (AGT) M235T polymorphism and angiotensin-converting-enzyme (ACE) intron
16 insertion/deletion (I/D) polymorphism among Korean preeclampsia women. We performed
a hospital-based case–control study on Korean women to investigate the association
between preeclampsia and the angiotensinogen M235T polymorphism and also to determine
the association between preeclampsia and the angiotensin-converting-enzyme intron
16 polymorphism. Methods: DNA was extracted from whole blood of 104 preeclampsia patients and 114 healthy
pregnant women. All samples were genotyped for all the polymorphisms using amplification
after PCR of known allelic variants. Results were analyzed with the chi-square test,
Student’s t-test, and logistic regression. Results: 18 of 50 women with preeclampsia (36.0%) in nulliparous women and 15 of 37 women
with preeclampsia (40.5%) in parous women were homozygous for methionine (M235) to
threonine (T235) substitution at residue 235 of AGT gene, versus 12 of 38 women in
nulliparous control women and 18 of 50 women in parous control women. There was no
association between the AGT M235T polymorphism and preeclampsia according to age.
Fourteen of 55 women with preeclampsia (25.5%) in nulliparous women and 11 of 39 women
with preeclampsia (28.2%) in parous women were homozygous for the D allele of the
ACE intron 16, versus 9 of 52 women in nulliparous control women and 16 of 53 women
in parous control women. No association was demonstrated between D allele of ACE intron
16 and preeclampsia according to age. There were significant differences in birth
weight and delivery weeks between controls and preeclampsia patients (P<0.001). There were no significant differences in age and nulliparity between controls
and preeclampsia patients. Conclusion: The result indicates that the AGT M235T polymorphism and the ACE intron 16 polymorphism
play no significant role in preeclampsia observed in Korean women.
Keywords
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Article info
Publication history
Accepted:
January 14,
2004
Received in revised form:
August 29,
2003
Identification
Copyright
© 2004 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
