Objective: The contribution of genetic factors to preeclampsia has been well documented. However, there has not been any study done on the association between preeclampsia and the angiotensinogen (AGT) M235T polymorphism and angiotensin-converting-enzyme (ACE) intron 16 insertion/deletion (I/D) polymorphism among Korean preeclampsia women. We performed a hospital-based case–control study on Korean women to investigate the association between preeclampsia and the angiotensinogen M235T polymorphism and also to determine the association between preeclampsia and the angiotensin-converting-enzyme intron 16 polymorphism. Methods: DNA was extracted from whole blood of 104 preeclampsia patients and 114 healthy pregnant women. All samples were genotyped for all the polymorphisms using amplification after PCR of known allelic variants. Results were analyzed with the chi-square test, Student’s t-test, and logistic regression. Results: 18 of 50 women with preeclampsia (36.0%) in nulliparous women and 15 of 37 women with preeclampsia (40.5%) in parous women were homozygous for methionine (M235) to threonine (T235) substitution at residue 235 of AGT gene, versus 12 of 38 women in nulliparous control women and 18 of 50 women in parous control women. There was no association between the AGT M235T polymorphism and preeclampsia according to age. Fourteen of 55 women with preeclampsia (25.5%) in nulliparous women and 11 of 39 women with preeclampsia (28.2%) in parous women were homozygous for the D allele of the ACE intron 16, versus 9 of 52 women in nulliparous control women and 16 of 53 women in parous control women. No association was demonstrated between D allele of ACE intron 16 and preeclampsia according to age. There were significant differences in birth weight and delivery weeks between controls and preeclampsia patients (P<0.001). There were no significant differences in age and nulliparity between controls and preeclampsia patients. Conclusion: The result indicates that the AGT M235T polymorphism and the ACE intron 16 polymorphism play no significant role in preeclampsia observed in Korean women.
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- Molecular basis of human hypertension: the role of angiotensin.Bailliere’s Clin Endocrinol Metab. 1995; 9: 657-678
- A study of angiotensin II pressor response throughout primigravid pregnancy.J. Clin Invest. 1973; 52: 2682-2689
- A molecular variant of angiotensinogen associated with preeclampsia.Nat Genet. 1993; 4: 59-61
- Angiotensinogen: a candidate gene involved in preeclampsia.Nat Genet. 1993; 4: 114-115
- Association of a variant of the angiotensinogen gene with pure type of hypertension in pregnancy in the Japanese: implication of a racial difference and significance of an age factor.Am. J. Med. Genet. 1999; 86: 232-236
- Maternal and fetal angiotensinogen gene allele sharing in preeclampsia.Br J Obstet Gynecol. 1999; 106: 244-251
- Angiotensinogen gene variation in a population case–control study of preeclampsia/eclampsia in Australians and Chinese.Electrophoresis. 1997; 18: 1646-1649
- The relation of angiotensin-converting enzyme to the pregnancy-induced hypertension-preeclampsia syndrome.Am. J. Obstet Gynecol. 1986; 154: 792-800
- The influence of normotensive pregnancy and preeclampsia on angiotensin-converting enzyme.Acta Obstet Gynecol Scand. 1983; 62: 341-344
- Association of a polymorphism of the angiotensin I-converting enzyme gene with essential hypertension.Biochem Biophy Res Commun. 1992; 184: 9-15
- A common genetic variant of the angiotensin converting enzyme is associated with both preeclampsia and chronic hypertension.Am. J. Obstet Gynecol. 1995; 172 ([abstract]): 374
- Angiotensin-converting enzyme insertion-deletion polymorphism in normotensive and preeclamptic pregnancies.J. Hypertens. 1999; 77: 765-768
- Hypertension, serum angiotensinogen, and molecular variants of the angiotensinogen gene among Nigerians.Circulation. 1997; 95: 2348-2350
- The serum angiotensinogen concentration and variants of the angiotensinogen gene in white and black children.J. Clin. Invest. 1995; 95: 948-953
- Effect of angiotensin-converting enzyme gene polymorphism on pregnancy outcome, enzyme activity and zinc concentration.Obstet Gynecol. 1996; 88: 497-502
- The plasma renin–angiotensin system in preeclampsia: effects of magnesium sulfate.Obstet Gynecol. 1989; 73: 934-937
- Serum angiotensin-converting enzyme activity in pregnancy-induced hypertension.Gynecol Obstet Invest. 1992; 33: 138-141
- Renin-angiotensin-aldosterone system in pregnancy-induced hypertension.J. Hum Hypertens. 2000; 14: s36-s39
- Genetic variation of the angiotensin-converting enzyme gene: increased frequency of the insertion allele in Koreans.Clin Genet. 1997; 51: 35-38
Accepted: January 14, 2004
Received in revised form: August 29, 2003
© 2004 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.