Abstract
Objective: To analyze retrospectively the effect of cryopreservation on donor’s sperm. Study design: Data were collected on 178 cryopreserved-thawed sperm specimens from 44 donors and
624 oocytes from 62 women, which underwent in vitro fertilization-embryo transfer
(IVF-ET) treatments with donor’s sperm. Data on fresh sperm, 175 sperm specimens from
76 couples which underwent IVF-ET treatments, were used as a control group. Semen
analysis was done by cell concentration, percent of motility, and quality of motility
according to the World Health Organization (WHO) recommendation. Sperm quality parameters
which had the strongest impact on fertilization capacity were determined using the
statistical response surface model and conjoint analysis. Results: Passing sperm through Percoll column decreased sperm concentration, with no improvement
in sperm motility but with a slight increase in quality of motility. Quality of motility
of donor’s sperm had the strongest impact on fertilization capacity. Conclusion: Current freezing–thawing protocols of sperm cause a decrease in sperm parameters
without affecting fertilization capacity. Furthermore, quality of motility of frozen–thawed
sperm seems to be a significant measure of sperm fertilization capacity.
Keywords
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Article info
Publication history
Accepted:
January 14,
2004
Received in revised form:
October 27,
2003
Received:
January 10,
2003
Identification
Copyright
© 2004 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
