Abstract
Objective
The objective was to assess the effectiveness of the newly developed immunomodulator
RESAN in the prophylaxis and treatment of endometriosis induced in rats.
Study design
The study was performed on 58 Wistar rats. Twelve weeks before endometriosis induction,
the RESAN vaccine was administered to 24 rats (100 mg i.m. and 100 mg s.c.). Endometriosis induction was performed in 48 rats, which were divided into
two groups: group I, the prophylaxis group, consisting of 24 previously vaccinated
rats; and group II, the treatment group, comprising the other 24 rats, which had not
been vaccinated. The graft (4 mm × 4 mm) of endometrium was attached to the parietal peritoneum. A sham operation was performed
in 10 rats (group III). After 3 months, a second laparotomy was performed in all animals,
and endometriotic foci were excised when present. RESAN was administered to the group
II animals. After an additional 3 months, a third laparotomy was performed in all
animals of the three groups.
Results
Positive, histologically confirmed endometriosis was found in 4.3% of the animals
in group I and in 69.6% of group II rats (p < 0.0001). Macroscopic assessment revealed endometriosis in 21.7% and 91.3% of animals
in groups I and II, respectively (p < 0.0001). At final laparotomy, 3 months after excision of the previously suspected
foci, no signs of endometriosis were found according to both macroscopic assessment
and histological examination. During the second laparotomy intraperitoneal adhesions
were present in 13.0% of the animals in group I and in 61.0% of those in group II.
No adhesions were present in group III. At the final laparotomy, the adhesions were
present in only three of the animals in group II (p < 0.0009).
Conclusions
RESAN seems to be effective in both the prophylaxis and treatment of endometriosis,
as well as in the prophylaxis of adhesions. Histological confirmation of endometriosis
should be mandatory.
Keywords
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Article info
Publication history
Published online: November 12, 2008
Accepted:
September 4,
2008
Received in revised form:
May 13,
2008
Received:
January 6,
2008
Identification
Copyright
© 2008 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
