Abstract
Objective
The objective was to assess the effectiveness of the newly developed immunomodulator
RESAN in the prophylaxis and treatment of endometriosis induced in rats.
Study design
The study was performed on 58 Wistar rats. Twelve weeks before endometriosis induction,
the RESAN vaccine was administered to 24 rats (100 mg i.m. and 100 mg s.c.). Endometriosis induction was performed in 48 rats, which were divided into
two groups: group I, the prophylaxis group, consisting of 24 previously vaccinated
rats; and group II, the treatment group, comprising the other 24 rats, which had not
been vaccinated. The graft (4 mm × 4 mm) of endometrium was attached to the parietal peritoneum. A sham operation was performed
in 10 rats (group III). After 3 months, a second laparotomy was performed in all animals,
and endometriotic foci were excised when present. RESAN was administered to the group
II animals. After an additional 3 months, a third laparotomy was performed in all
animals of the three groups.
Results
Positive, histologically confirmed endometriosis was found in 4.3% of the animals
in group I and in 69.6% of group II rats (p < 0.0001). Macroscopic assessment revealed endometriosis in 21.7% and 91.3% of animals
in groups I and II, respectively (p < 0.0001). At final laparotomy, 3 months after excision of the previously suspected
foci, no signs of endometriosis were found according to both macroscopic assessment
and histological examination. During the second laparotomy intraperitoneal adhesions
were present in 13.0% of the animals in group I and in 61.0% of those in group II.
No adhesions were present in group III. At the final laparotomy, the adhesions were
present in only three of the animals in group II (p < 0.0009).
Conclusions
RESAN seems to be effective in both the prophylaxis and treatment of endometriosis,
as well as in the prophylaxis of adhesions. Histological confirmation of endometriosis
should be mandatory.
Keywords
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References
- Endometriosis 1991: a discussion document.Hum Reprod. 1992; 7: 423-435
- Cytokine profiles in autologous peritoneal fluid and peripheral blood of women with deep and superficial endometriosis.Arch Gynecol Obstet. 2001; 265: 40-44
- Was Sampson wrong?.Fertil Steril. 2002; 78: 686-693
- Is endometriosis an endometrial disease?.Eur J Obstet Gynecol Reprod Biol. 2000; 91: 113-125
- Epidemiology of endometriosis.J Epidemiol Community Health. 1993; 47: 84-88
- Extracts from the “clinical evidence” Endometriosis.BMJ. 2000; 320: 1449-1452
- Endometriosis and subfertility: is the relationship resolved?.Semin Reprod Med. 2003; 21: 243-254
- Adjuvant active specific immunotherapy for stage II and III colon cancer with an autologous tumor cell vaccine: Eastern Cooperative Oncology Study (E5283).J Clin Oncol. 2000; 58: 215-218
- Immunization of cancer patients with a HER-2/neu, HLA-A2 peptide, p369-377, results in short-lived peptide-specific immunity.Clin Cancer Res. 2002; 8: 1014-1018
- Effect of BCG vaccine on peritoneal endometriotic implants in a rat model of endometriosis.Aus NZ J Obstet Gynecol. 2006; 46: 38-41
- BCG vaccination to prevent implantation of endometriosis: an experimental study in rats.Eur J Obstet Gynecol Reprod Biol. 2001; 98: 209-212
- Pentoxifylline versus placebo in the treatment of infertility associated with minimal or mild endometriosis: a pilot randomized clinical trial.Hum Reprod. 1997; 12: 2046-2050
Yantchenko VV, Yantchenko AV, Yantchenko LK. Imitators of the tumor antigens. The patent committee RB, appl. N970547; 1997.
- The influence of simultaneous whole body exposure to cigarette smoke and low-protein diet feeding on fertility, reproduction and progeny of rats.Hum Exp Toxicol. 2002; 21: 615-621
- [ekspermantalny model endometriozy u szczurów] The experimental model of endometriosis in rats.Ginek Pol. 1990; 61: 387-391
- Integrin pattern in rats endometrium after endometriosis induction.Pol J Gynaecol Invest. 2002; 5: 293-298
- Integrin pattern in rats endometrium after endometriosis excision.Pol J Gynaecol Invest. 2002; 5: 299-302
- Apoptosis expression in rats’ endometrium after surgical induction of endometriosis.Ginek Pol. 2003; 74: 262-266
The patent committee of Republic of Belarus, No. 5942; 1997.
- Immunology of endometriosis.Best Pract Res Clin Obstet Gynaecol. 2004; 18: 245-263
- Apoptosis pattern in human endometrium in women with pelvic endometriosis.Eur J Obstet Gynecol Reprod Biol. 2007; 132: 107-110
- Adjuvant active specific immunotherapy for human colorectal cancer: 6, 5-year median follow-up of phase III prospective randomized trial.J Clin Oncol. 1993; 11: 390-399
- Human high molecular weight melanoma-associated antigen (HMW-MAA) mimicry by mouse anti-idiotypic monoclonal antibody MK2-23: induction of humoral anti-HMW-MAA immunity and prolongation of survival in patients with stage IV melanoma.Proc Natl Acad Sci USA. 1992; 15: 466-470
- Effect of DETOX as an adjuvant for melanoma vaccine.Vaccine. 1995; 13: 503-508
- GM2-KLH conjugate vaccine: increased immunogenicity in melanoma patients after administration with immunological adjuvant QS-21.Cancer Res. 1995; 55: 2783-2788
Article info
Publication history
Published online: November 12, 2008
Accepted:
September 4,
2008
Received in revised form:
May 13,
2008
Received:
January 6,
2008
Identification
Copyright
© 2008 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.