Abstract
Objectives
This study aimed to detect the presence and prevalence of HPV-DNA in the cervical
swab samples obtained from patients with cervical cancer, premalignant cervical lesions
and benign cervical smear results, and to identify the potential risk factors influencing
this prevalence.
Study design
Smear preparations were examined and classified according to the Bethesda system.
HPV-DNA detection and genotyping was carried out using polymerase chain reaction combined
with reverse hybridization line-probe assays. Age, smoking habit, age at first sexual
intercourse, number of sexual partners, number of term births, contraceptive method,
progesterone therapy, history of sexually transmitted diseases, history or existence
of warts, existence of cervical infection and the history of circumcision of male
sexual partners were recorded.
Results
Six hundred and forty-two women (96 women with abnormal cervical cytology and 546
women with normal cytology) provided cervical samples. Multiplex PCR testing revealed
that prevalence of HPV-DNA was 38.9% in our study population. HPV-DNA was detected
in 78.3% of the women with cervical cancer and 76.9% of the women with HGSIL. Abnormal
cervical cytology was observed in 30% of HPV-DNA positive cases and in 5.4% of HPV-DNA
negative cases. Our findings also indicate that smoking habit, number of sexual partners,
history of sexually transmitted diseases, and abnormal cervical cytology were associated
with HPV infection. With respect to parity, there was a decreased risk of HPV infection
with the increase in the number of births.
Conclusions
Estimates of the prevalence of HPV infection vary greatly around the world, so the
factors that contribute to the rare occurrence of cervical cancer after HPV infection
might also differ from country to country. Information gathered from this study could
be used to prioritize limited screening and treatment services given to woman who
have specific characteristics that may put them at an increased risk of HPV disease.
Keywords
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Article info
Publication history
Published online: June 27, 2011
Accepted:
June 9,
2011
Received in revised form:
April 7,
2011
Received:
February 2,
2011
Identification
Copyright
© 2011 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
