Abstract
Objective
The aim of the present work was to look at differences in the placental tissue expression
of KiSS-1 and REN genes from preeclamptic and healthy pregnant women, that could account
for a possible synergistic function for both genes in the pathogenesis of preeclampsia.
Study design
This case–control study involved 27 preeclamptic women and 27 normoevolutive pregnant
women. cDNA was obtained from placental tissue to carry out qPCR for both KiSS-1 and
REN genes in order to compare mRNA expression levels in the studied groups. Statistical
analysis showed expression differences that correlate with clinical and/or biochemical
variables.
Results
Higher expression for KiSS-1 in PEE vs. control woman (p = 0.001) was observed, whereas no difference was observed for REN expression (p = 0.300) when all the subjects were included. However, REN expression was significant
higher when the samples were stratified according to preeclampsia severity. For 18
mild preeclamptic patients the p-value was p = 0.001 compared to their controls, while for the remaining nine with severe preeclampsia
the expression became significant (p = 0.001).
Conclusion
Our results suggest that the high KiSS-1 expression seen in preeclamptic patients
is in accordance with its role as an inhibitor of trophoblast invasiveness and maintained
until the end of gestation. On the other hand, aggressive therapeutic management and/or
severity status of patients have a direct effect on placental REN expression levels,
masking the natural high expression of this gene on preeclamptic placental tissue.
Therefore it was not possible to establish a real concordant expression profile for
KiSS-1 and REN genes.
Keywords
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Article info
Publication history
Published online: July 28, 2011
Accepted:
July 11,
2011
Received in revised form:
July 4,
2011
Received:
March 8,
2011
Identification
Copyright
© 2011 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
