Abstract
Objective To evaluate the effect of carbon dioxide (CO2) pneumoperitonal pressure on the ultrastructure of implanted endometriotic lesions
in a rat model.
Study design An endometriotic model was established in Sprague-Dawley rats with peritoneal implantation
of their own endometrial tissue to form ectopic endometrial tissue. Thirty rats were
divided at random into three groups: control group, 10 mmHg CO2 pneumoperitoneum group and 20 mmHg CO2 pneumoperitoneum group. CO2 inflation lasted for 1 h in both pneumoperitoneum groups. Selected endometriotic lesions were examined by
electron microscopy 1 week after CO2 pneumoperitoneum to determine changes in the ultrastructure of cell organelles of
glandular and stromal cells.
Results In the control group, stromal cells had an orderly arrangement, and the structure
of the microvilli was normal. The 10 mmHg and 20 mmHg CO2 pneumoperitoneum groups had increased chromatin margination of glandular epithelial
cells, reduced or absent mitochondrial cristae and mitochondrial swelling. Further
ultrastructural damage included reduced or absent microvilli, widened or collapsed
cell junctions, and stromal cells with a disorderly arrangement. Ultrastructural damage
of glandular epithelial cells was more severe in the 20 mmHg group compared with the 10 mmHg group.
Conclusion CO2 pneumoperitoneum leads to significant ultrastructural damage of endometriotic lesions.
Higher CO2 pneumoperitoneal pressure resulted in more damage than lower CO2 pneumoperitoneal pressure.
Keywords
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Article info
Publication history
Published online: September 09, 2013
Accepted:
August 23,
2013
Received in revised form:
June 13,
2013
Received:
December 26,
2012
Identification
Copyright
© 2013 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
