Iatrogenic preterm prelabour rupture of fetal membranes (iPPROM) remains the main complication after invasive interventions into the intrauterine cavity. The aim of this study was to evaluate the sealing capability and tissue interaction of mussel-mimetic tissue adhesive (mussel glue) in comparison to fibrin glue on punctured fetal membranes in vivo.
A mid-gestational rabbit model was used for testing the materials. The fetal sacs of pregnant rabbits at day 23 were randomly assigned into experimental groups: unoperated (negative control), unclosed puncture (positive control), commercially available fibrin glue (FG) with decellularized amnion scaffold (DAM), mussel glue (MG) with DAM, or mussel glue alone. Evaluation was done at term (30 days’ gestation) assessing fetal survival, fetal membrane integrity and histology of the membranes.
Fetal survival was not significantly lower in any of the treatment groups compared to the negative control. All plugging materials could be found at the end of the pregnancy and no adverse effects on the fetus or the pregnant does could be observed. Sac integrity was higher in all treatment groups compared to the positive control group but significant only in the FG + DAM group. Cellular infiltration could be seen in fibrin glue and DAM in contrast to mussel glue which was only tightly adhering to the surrounding tissue. These cells were mostly of mesenchymal phenotype staining positive for vimentin. CD68 positive macrophages were found clustered around all the plugging materials, but their numbers were only significantly increased for the mussel glue alone group compared to negative controls.
Mussel glues performance in sealing fetal membranes in the rabbit model was comparable to that of fibrin glue. Taking into account its other favorable properties, it is a noteworthy candidate for a clinically applicable fetal membrane sealant.
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- Endoscopic laser surgery versus serial amnioreduction for severe twin-to-twin transfusion syndrome.N Engl J Med. 2004; 351: 136-144
- A randomized trial of prenatal versus postnatal repair of myelomeningocele.N Engl J Med. 2011; 364: 993-1004
- Preterm prelabor rupture of membranes and fetal survival after minimally invasive fetal surgery: a systematic review of the literature.Fetal Diagn Ther. 2011; 31: 1-9
- A histological study of fetoscopic membrane defects to document membrane healing.Placenta. 2006; 27: 452-456
- Enhancing sealing of fetal membrane defects using tissue engineered native amniotic scaffolds in the rabbit model.Am J Obstet Gynecol. 2007; 196: 261-267
- Fetoscopic closure of punctured fetal membranes with acellular human amnion plugs in a rabbit model.Obstet Gynecol. 2007; 110: 1121-1129
- Bioengineering of foetal membrane repair.Swiss Med Wkly. 2008; 138: 596-601
- Mussel-mimetic tissue adhesive for fetal membrane repair: a standardized ex vivo evaluation using elastomeric membranes.Prenat Diagn. 2011; 31: 654-660
- Mussel-mimetic tissue adhesive for fetal membrane repair: an ex vivo evaluation.Acta Biomater. 2012; 8: 4365-4370
- Fetal membrane patch and biomimetic adhesive coacervates as a sealant for fetoscopic defects.Acta Biomater. 2012; 8: 2160-2165
- Biological performance of mussel-inspired adhesive in extrahepatic islet transplantation.Biomaterials. 2010; 31: 420-427
- Injectable candidate sealants for fetal membrane repair: bonding and toxicity in vitro.Am J Obstet Gynecol. 2010; 202: 8581-8589
- The midgestational rabbit as a model for the creation of membrane defects after needle fetoscopy.Am J Obstet Gynecol. 1999; 180: 1263-1267
- Synthesis and gelation of DOPA-modified poly(ethylene glycol) hydrogels.Biomacromolecules. 2002; 3: 1038-1047
- Applications of fibrin sealant in surgery.Surg Innov. 2005; 12: 203-213
- NIH Image to ImageJ: 25 years of image analysis.Nat Methods. 2012; 9: 671-675
- Macrophage phenotype as a determinant of biologic scaffold remodeling.Tissue Eng A. 2008; 14: 1835-1842
Published online: September 16, 2013
Accepted: September 2, 2013
Received in revised form: July 31, 2013
Received: March 3, 2013
☆This work was supported by the Swiss National Fund (3200B-124925/1), European Community's EuroStec Programme (contract 037409) and European Community's Seventh Framework Programme MultiTERM (grant agreement nr. 238551).
© 2013 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.