Abstract
Objective
Iatrogenic preterm prelabour rupture of fetal membranes (iPPROM) remains the main
complication after invasive interventions into the intrauterine cavity. The aim of
this study was to evaluate the sealing capability and tissue interaction of mussel-mimetic
tissue adhesive (mussel glue) in comparison to fibrin glue on punctured fetal membranes
in vivo.
Study design
A mid-gestational rabbit model was used for testing the materials. The fetal sacs
of pregnant rabbits at day 23 were randomly assigned into experimental groups: unoperated
(negative control), unclosed puncture (positive control), commercially available fibrin
glue (FG) with decellularized amnion scaffold (DAM), mussel glue (MG) with DAM, or
mussel glue alone. Evaluation was done at term (30 days’ gestation) assessing fetal
survival, fetal membrane integrity and histology of the membranes.
Results
Fetal survival was not significantly lower in any of the treatment groups compared
to the negative control. All plugging materials could be found at the end of the pregnancy
and no adverse effects on the fetus or the pregnant does could be observed. Sac integrity
was higher in all treatment groups compared to the positive control group but significant
only in the FG + DAM group. Cellular infiltration could be seen in fibrin glue and DAM in contrast
to mussel glue which was only tightly adhering to the surrounding tissue. These cells
were mostly of mesenchymal phenotype staining positive for vimentin. CD68 positive
macrophages were found clustered around all the plugging materials, but their numbers
were only significantly increased for the mussel glue alone group compared to negative
controls.
Conclusions
Mussel glues performance in sealing fetal membranes in the rabbit model was comparable
to that of fibrin glue. Taking into account its other favorable properties, it is
a noteworthy candidate for a clinically applicable fetal membrane sealant.
Keywords
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Article info
Publication history
Published online: September 16, 2013
Accepted:
September 2,
2013
Received in revised form:
July 31,
2013
Received:
March 3,
2013
Footnotes
☆This work was supported by the Swiss National Fund (3200B-124925/1), European Community's EuroStec Programme (contract 037409) and European Community's Seventh Framework Programme MultiTERM (grant agreement nr. 238551).
Identification
Copyright
© 2013 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
