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The effect of hepatitis B virus infected embryos on pregnancy outcome

  • Feng Ye
    Affiliations
    Department of Infectious Diseases, the First Affiliated Hospital, Xi’an Jiaotong University, Xi’an Yanta Road No. 277, Xi’an 710061, Shaanxi Province, China
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  • Yi Liu
    Correspondence
    Corresponding author. Tel.: +86 029 85324132; fax: +86 029 5252812.
    Affiliations
    Department of Traditional Chinese Medicine, the First Affiliated Hospital, Xi’an Jiaotong University, Xi’an Yanta Road No. 277, Xi’an 710061, Shaanxi Province, China
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  • Yan Jin
    Affiliations
    Department of Gastroenterology, Shaanxi Provincial People's Hospital, Youyixi Road, 256#, Xi’an 710068, Shaanxi Province, China
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  • Juanzi Shi
    Affiliations
    Department of Reproductive and Endocrinology, Shaanxi Maternal and Child Health Hospital, Houzaimen, 73#, Xi’an 710003, Shaanxi Province, China
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  • Xueliang Yang
    Affiliations
    Department of Infectious Diseases, the First Affiliated Hospital, Xi’an Jiaotong University, Xi’an Yanta Road No. 277, Xi’an 710061, Shaanxi Province, China
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  • Xiaojing Liu
    Affiliations
    Department of Infectious Diseases, the First Affiliated Hospital, Xi’an Jiaotong University, Xi’an Yanta Road No. 277, Xi’an 710061, Shaanxi Province, China
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  • Xi Zhang
    Affiliations
    Department of Infectious Diseases, the First Affiliated Hospital, Xi’an Jiaotong University, Xi’an Yanta Road No. 277, Xi’an 710061, Shaanxi Province, China
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  • Shumei Lin
    Affiliations
    Department of Infectious Diseases, the First Affiliated Hospital, Xi’an Jiaotong University, Xi’an Yanta Road No. 277, Xi’an 710061, Shaanxi Province, China
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  • Ying Kong
    Affiliations
    Department of Infectious Diseases, the First Affiliated Hospital, Xi’an Jiaotong University, Xi’an Yanta Road No. 277, Xi’an 710061, Shaanxi Province, China
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  • Linshu Zhang
    Affiliations
    Department of Infectious Diseases, the First Affiliated Hospital, Xi’an Jiaotong University, Xi’an Yanta Road No. 277, Xi’an 710061, Shaanxi Province, China
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Published:October 28, 2013DOI:https://doi.org/10.1016/j.ejogrb.2013.10.002

      Abstract

      Objective

      HBV vertical transmission is the main reason for chronic HBV infection, but there is no clear conclusion about the effect of HBV infection on pregnancy outcome. This study aimed to investigate the effect of HBV-infected embryos on pregnancy outcome.

      Study design

      75 couples who received ART treatment were followed up. Among them were 25 couples (group A) with at least the husband or wife infected with HBV, and 50 couples (group B) with negative serum HBV markers of both husband and wife. The clinical pregnancy rate, early abortion rate, neonatal malformation rate, neonatal HBV infection rate, and HBV infection rate of the six-month-old infant were compared between these two groups, and Fisher's exact test was applied. HBV mRNA in abandoned embryos of the patients was detected by single-cell RT-PCR.

      Results

      Both the early abortion rate and the abortion rate with the mother infected HBV in group A were significantly higher than in group B (P = 0.043, P = 0.030 respectively). The clinical pregnancy rates of groups A and B were 36% and 44% respectively (P = 0.248 > 0.05). The neonatal malformation rate, neonatal HBV infection rate, and HBV infection rate of six-month-old infants in group A and group B were all 0. Specific HBV mRNA fragments were detected in 6 cases of 62 cleavage embryos in group A, giving a positive rate of 9.7%. The positive rates with mother infected and father infected with HBV were 13.2% (5/38) and 5.6% (1/18) respectively.

      Conclusions

      HBV infection can increase the early abortion rate of pregnancy, and the reason may be related to HBV-infected embryos.

      Keywords

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