Abstract
Objective
The aim of this study was to investigate the effect of infliximab on experimentally
induced ovarian ischemia/reperfusion injury (IRi).
Study design
A total of 42 female rats were equally divided into 6 experimental groups; group 1:
sham operation, group 2: 3-h ischemia, group 3 and 4: 3-h ischemia, 3-h reperfusion,
group 5 and 6: 3-h ischemia, 24 h reperfusion. In group 4 and group 6, 30 min before reperfusion, infliximab was administered intraperitoneally at a dose of
5 mg/kg. Bilateral ovaries were removed for histopathologic and biochemical analysis.
Serum MDA (sMDA), tissue MDA (tMDA), serum NO (sNO), tissue NO (tNO) and serum catalase
concentrations were analyzed. Tissue damage of ovarian tissue was scored by histological
examination.
Results
The infliximab administration significantly lowered the sNO, tNO and sMDA concentrations
in group 4 compared to group 3 (p = 0.041, p = 0.025 and p = 0.035, respectively). sNO, tNO and sMDA concentrations were also lower in group 6
when compared to group 5, but this differences were not significant (p > 0.05). On the other hand, tMDA concentrations were lower in infliximab-applied groups
when compared to ischemia/reperfusion groups (group 3 vs. 4 and 5 vs. 6) (p = 0.045 and p = 0.048, respectively). Moreover, histopathologic tissue damage scores in infliximab
administration groups were significantly lower than in ischemia/reperfusion groups
(p < 0.001).
Conclusion
Infliximab attenuates I/R-induced ovarian tissue injury in rats subjected to ischemia/reperfusion.
Keywords
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Article info
Publication history
Published online: October 17, 2013
Accepted:
September 29,
2013
Received in revised form:
August 21,
2013
Received:
June 19,
2013
Identification
Copyright
© 2013 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
