Food intake diet and sperm characteristics in a blue zone: a Loma Linda Study



      The study examined the effect the life-long vegetarian diet on male fertility and focused on vegetarians living in the Loma Linda blue zone, a demographic area known for life longevity. The objective was to compare sperm characteristics of vegetarian with non-vegetarian males.

      Study design

      The cross-sectional observational study was based on semen analyses of 474 males from 2009 to 2013. Patients categorized themselves as either life-long lacto-ovo vegetarians (N = 26; vegetable diet with dairy and egg products), vegans (N = 5; strictly vegetables with no animal products) or non-vegetarians (N = 443; no diet restrictions). Sperm quality was assessed using a computer-aided sperm analyzer and strict morphology and chromatin integrity were manually evaluated.


      Lacto-ovo vegetarians had lower sperm concentration (50.7 ± 7.4 M/mL versus non-vegetarians 69.6 ± 3.2 M/mL, mean ± S.E.M.). Total motility was lower in the lacto-ovo and vegan groups (33.2 ± 3.8% and 51.8 ± 13.4% respectively) versus non-vegetarians (58.2 ± 1.0%). Vegans had lowest hyperactive motility (0.8 ± 0.7% versus lacto-ovo 5.2 ± 1.2 and non-vegetarians 4.8 ± 0.3%). Sperm strict morphologies were similar for the 3 groups. There were no differences in rapid progression and chromatin integrity.


      The study showed that the vegetables-based food intake decreased sperm quality. In particular, a reduction in sperm quality in male factor patients would be clinically significant and would require review. Furthermore, inadequate sperm hyperactivation in vegans suggested compromised membrane calcium selective channels. However, the study results are cautiously interpreted and more corroborative studies are needed.


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        • Fjerbaek A.
        • Knudsen U.B.
        Endometriosis, dysmenorrhea and diet – what is the evidence.
        Eur J Obstet Gynecol Reprod Biol. 2007; 132: 140-147
        • Murabayashi N.
        • Sugiyama T.
        • Zhang L.
        • et al.
        Maternal high-fat diets cause insulin resistance through inflammatory changes in fetal adipose tissue.
        Eur J Obstet Gynecol Reprod Biol. 2013; 169: 39-44
        • Bouvard V.
        • Loomis D.
        • Guyton K.Z.
        • et al.
        Carcinogenicity of consumption of red and processed meat: International Agency for Research on Cancer Monograph Working Group.
        Lancet Oncol. 2015; (pii:S1470-2045(15)00444-1)
        • Orlich M.J.
        • Singh P.N.
        • Sabaté J.
        • et al.
        Vegetarian dietary patterns and mortality in adventist health study 2.
        JAMA Int Med. 2013; 173: 1230-1238
        • Wong W.Y.
        • Zielhuis G.A.
        • Thomas C.M.
        • Merkus H.M.
        • Steegers-Theunissen R.P.
        New evidence of the influence of exogenous and endogenous factors on sperm count in man.
        Eur J Obstet Gynecol Reprod Biol. 2003; 110: 49-54
        • West M.C.
        • Anderson L.
        • McClure N.
        • Lewis S.E.
        Dietary oestrogens and male fertility potential.
        Hum Fert (Cambr). 2005; 8: 197-207
        • Liu Z.H.
        • Kanjo Y.
        • Mizutani S.
        A review of phytoestrogens: their occurrence and fate in the environment.
        Water Res. 2010; 44: 567-577
        • Chavarro J.E.
        • Toth T.L.
        • Sadio S.M.
        • Hauser R.
        Soy food and isoflavone intake in relation to semen quality parameters among men from an infertility clinic.
        Hum Reprod. 2008; 23: 2584-2590
        • Buettner D.
        The blue zones: lessons for living longer from the people who’ve lived the longest.
        978-1-4262-0400-5 National Geographic Books, Washington, DC2009, April 21 (OCLC 246886564)
        • Fraser G.E.
        Associations between diet and cancer, ischemic heart disease, and all-cause mortality in non-Hispanic white California Seventh-day Adventists.
        Am J Clin Nutr. 1999; 70: 532S-538S
        • World Health Organization
        WHO laboratory manual for the examination and processing of human semen.
        4th ed. World Health Organization, Geneva1999
        • Montgomery S.
        • Herring P.
        • Yancey A.
        • et al.
        Comparing self-reported disease outcomes, diet, and lifestyles in a national cohort of black and white Seventh-day Adventists.
        Prev Chronic Dis. 2007; 4: A62
        • Butler T.L.
        • Fraser G.E.
        • Beeson W.L.
        • et al.
        Cohort profile: the Adventist Health Study-2 (AHS-2).
        Int J Epidemiol. 2008; 37: 260-265
        • Balli K.S.
        • Patton W.C.
        • Jacobson J.D.
        • Corselli J.
        • King A.
        • Chan P.J.
        Sperm velocity in seminal plasma and the association with gender of offspring.
        Arch Androl. 2004; 50: 37-40
        • Kruger T.F.
        • Menkveld R.
        • Stander F.S.H.
        • et al.
        Sperm morphologic features as a prognostic factor in in vitro fertilization.
        Fertil Steril. 1986; 46: 1118-1123
        • Kruger T.F.
        • Acosta A.A.
        • Simmons K.F.
        • Swanson R.J.
        • Matta J.F.
        • Oehninger S.
        Predictive value of abnormal sperm morphology in in vitro fertilization.
        Fertil Steril. 1988; 49: 112-117
        • Burkman L.J.
        Characterization of hyperactivated motility by human spermatozoa during capacitation: comparison of fertile and oligozoospermic sperm populations.
        Arch Androl. 1984; 13: 153-165
        • Sousa A.P.
        • Tavares R.S.
        • Velez de la Calle J.F.
        • et al.
        Dual use of Diff-Quik-like stains for the simultaneous evaluation of human sperm morphology and chromatin status.
        Hum Reprod. 2009; 24: 28-36
        • Qi H.
        • Moran M.M.
        • Navarro B.
        • et al.
        All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility.
        Proc Nat Acad Sci U S A. 2007; 104: 1219-1223
        • Brini M.
        • Carafoli E.
        Calcium pumps in health and disease.
        Physiol Rev. 2009; 89: 1341-1378
        • Rozati R.
        • Reddy P.P.
        • Reddanna P.
        • Mujtaba R.
        Role of environmental estrogens in the deterioration of male factor fertility.
        Fertil Steril. 2002; 78: 1187-1194
        • Sharpe R.M.
        Lifestyle and environmental contribution to male infertility.
        Br Med Bull. 2000; 56: 630-642
        • Glover A.
        • Assinder S.J.
        Acute exposure of adult male rats to dietary phytoestrogens reduces fecundity and alters epididymal steroid hormone receptor expression.
        J Endocrinol. 2006; 189: 565-573
        • Casini M.L.
        • Gerli S.
        • Unfer V.
        An infertile couple suffering from oligospermia by partial sperm maturation arrest: can phytoestrogens play a therapeutic role? A case report study.
        Gynecol Endocrinol. 2006; 22: 399-401
        • Messina M.
        • Watanabe S.
        • Setchell K.D.
        Report on the 8th international symposium on the role of soy in health promotion and chronic disease prevention and treatment.
        J Nutr. 2009; 139: 796S-802S
        • Beaton L.K.
        • Dillingham B.L.
        • McVeigh B.L.
        • Lampe J.W.
        • Duncan A.
        Soy protein isolates of varying isoflavone content do not adversely affect semen quality in healthy young men.
        Fertil Steril. 2010; 94: 1717-1722
        • Setchell K.D.
        • Clerici C.
        Equol: history, chemistry, and formation.
        J Nutr. 2010; 140: 1355S-1362S
        • Aquila S.
        • Sisci D.
        • Gentile M.
        • et al.
        Estrogen receptor (ER) alpha and ER beta are both expressed in human ejaculated spermatozoa: evidence of their direct interaction with hosphatidylinositol-3-OH kinase/Akt pathway.
        J Clin Endocrinol Metabol. 2004; 89: 1443-1451
        • Chiu Y.H.
        • Afeiche M.C.
        • Gaskins A.J.
        • et al.
        Fruit and vegetable intake and their pesticide residues in relation to semen quality among men from a fertility clinic.
        Hum Reprod. 2015; 30: 1342-1351
        • Baker B.P.
        • Benbrook C.M.
        • Groth 3rd., E.
        • Lutz Benbrook K.
        Pesticide residues in conventional, integrated pest management (IPM)-grown and organic foods: insights from three US data sets.
        Food Addit Contam. 2002; 19: 427-446
        • Lu C.
        • Toepel K.
        • Irish R.
        • Fenske R.A.
        • Barr D.B.
        • Bravo R.
        Organic diets significantly lower children's dietary exposure to organophosphorus pesticides.
        Environ Health Persp. 2006; 114: 260-263
        • Juhler R.K.
        • Larsen S.B.
        • Meyer O.
        • et al.
        Human semen quality in relation to dietary pesticide exposure and organic diet.
        Arch Environ Contam Toxicol. 1999; 37: 415-423
        • Sinclair S.
        Male infertility: nutritional and environmental considerations.
        Altern Med Rev. 2000; 5 ([Review]): 28-38
        • Tonstad S.
        • Stewart K.
        • Oda K.
        • Batech M.
        • Herring R.P.
        • Fraser G.E.
        Vegetarian diets and incidence of diabetes in the Adventist Health Study-2.
        Nutr Metabol Cardiovasc Dis. 2013; 23: 292-299