Abstract
Objective
Kisspeptins are multifunctional peptides; it has been shown that they act as inhibitors
of tumor metastasis in a range of cancers and that they are also involved in cell
invasion through regulation of matrix metalloproteinases (MMPs). The aim of this study
was to investigate the expression of KISS-1 protein in adenomyosis lesions compared
with matched eutopic endometrium, as well as with endometrium from patients without
adenomyosis.
Study Design
In this comparative, non-interventional study, adenomyosis and corresponding eutopic
endometrium samples from women with histologically proven adenomyosis after hysterectomy,
and eutopic endometrium samples from women without adenomyosis were analysed. Expression
of KISS-1 protein was analyzed immunohistochemically in formalin-fixed, paraffin-embedded
adenomyotic tissue specimens (n = 29), matched eutopic endometrium from the same patients (n = 29) and normal endometrium from patients without adenomyosis (n = 29).
Results
Using a semi-quantitative immunohistochemical score, we found that KISS-1 protein
expression was higher in the adenomyotic as compared with matched eutopic glandular
endometrium (p < 0.05), in which in turn KISS-1 protein expression was higher than those from patients
without adenomyosis (p < 0.001). The inverse correlation was found in the stroma, between adenomyosis lesions
and matched eutopic endometrium (p < 0.01), while no statistically significant correlation was found in KISS-1 protein
expression in the stroma between patients with and without adenomyosis.
Conclusions
KISS-1 protein expression appears to be up-regulated in adenomyotic as compared with
eutopic glandular endometrium of patients with, as well as women without adenomyosis.
These findings are suggestive of the involvement of KISS-1 protein in the pathogenesis
and maintenance of adenomyosis. Future studies should investigate whether KISS1 protein
could be used as a marker for early and minimally invasive detection of adenomyosis,
based on its differential protein expression pattern in the eutopic endometrium of
patients with and without adenomyosis.
Keywords
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Article info
Publication history
Published online: December 05, 2016
Accepted:
December 3,
2016
Received in revised form:
November 27,
2016
Received:
June 30,
2016
Identification
Copyright
© 2016 Elsevier Ireland Ltd. All rights reserved.
