Abstract
Objective
To analyze the distribution and quantification of the key structural extracellular
matrix components of the urethral tissue in a rat model of hyperglycemia and pregnancy.
Study design
A total of 120 female Wistar rats were distributed into the following four experimental groups: virgin, pregnant,
hyperglycemic and hyperglycemic + pregnant groups. The urethra was harvested for histochemical,
morphometric, immunohistochemical, Western blot and glycosaminoglycan analyses. All
protocols were approved by the Institutional Animal Care and Use Committee of Botucatu
Medical School (process number 828–2010).
Results
The hyperglycemic + pregnant group showed significantly increased stiffness in urethral
tissue. The total striated muscle was decreased, with increased deposition of collagen
fibers around the muscle fibers and a change in the organization of the collagen fibrils.
An increase in the relative collagen type I/III ratio and a decrease in total glycosaminoglycans
were also observed.
Conclusions
This study provides the first line of experimental evidence supporting a metabolic
relationship between hyperglycemia and urethral remodeling of connective tissue in
pregnant rats. The different organization of the collagen fibrils and the profile
of glycosaminoglycans found in urethral samples suggest that the pathology of the
urethral fibromuscular system could be related to hyperglycemia-induced pelvic floor
dysfunction in women, which has direct clinical implications with the possibility
to develop new multidisciplinary treatments for improving the health care of these
women.
Keywords
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Article info
Publication history
Published online: December 16, 2017
Accepted:
December 15,
2017
Received in revised form:
December 14,
2017
Received:
June 19,
2017
Identification
Copyright
© 2017 Elsevier B.V. All rights reserved.
