Abstract
Objectives
Protein 16 (p16CDKN2A) and transforming growth factor-beta 1 (TGF- β1) are important tumor suppressor molecules.
The aim of this study was to evaluate the frequency and simultaneous expression of
p16CDKN2A and TGF- β1 in cervical intraepithelial neoplasia (CIN) and cervical cancer and their
relationship whit the neoplasia progression.
Study design
To evaluate the expressions of p16CDKN2A and TGF- β1 an immunohistochemical study of both proteins in 75 cervical tissues
(24 CIN I, 17 CIN II, 15 CIN III and 19 squamous cell cancer) was performed.
Results
Increased expression of epithelial and stromal p16CDKN2A in all grades of CIN and cancer was observed. Healthy controls were negative. The
frequency of p16CDKN2A expression in the patients was as follow: 75% in CIN I and 100% in CIN II, CIN III
and cancer. TGF- β1 expression was found increased in all patients with CIN I and
CIN II and decreased in CIN III and cancer; 60% of patients with CIN III and 16% with
cancer showed reactivity for TGF- β1. High intensity of p16CDKN2A reactivity and low intensity of TGF- β1 reactivity were observed.
Conclusions
The linear frequency of p16CDKN2A expression accompanied by decreased frequency of TGF- β1 in CIN III and cancer could
be involved in the neoplasia progression.
Keywords
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Article info
Publication history
Published online: July 21, 2018
Accepted:
July 20,
2018
Received in revised form:
July 18,
2018
Received:
June 1,
2018
Identification
Copyright
© 2018 Elsevier B.V. All rights reserved.
