Abstract
Objectives
To compare the effectiveness of sperm selection using cumulus oophorus complexes (COCs)
and conventional sperm preparation methods on sperm quality and DNA fragmentation
Methods
Normal semen samples under the World Health Organization (WHO)’s 2010 eligibility
criteria were collected and processed using conventional sperm preparation methods.
The prepared sperm were divided into two groups. Spermatozoa in the study group were
selected based on their ability to penetrate a layer of COCs. In the control group,
spermatozoa were kept in culture medium under similar conditions. The selected-sperm
were evaluated based on sperm quality and DNA fragmentation.
Results
Thirty normal semen samples were recruited. Spermatozoa that were able to passthrough
the COCs had significantly higher sperm motility parameters than the control group
(curvilinear velocity [VCL; 143.5 vs 122.2; P < 0.01], average path velocity [VAP; 83.6 vs 69.3; P < 0.01], straight-line velocity [VSL; 67.95 vs 60.45; P < 0.01]). The percentage of normal spermatozoa morphology in the COCs group was significantly
higher than in the control group (21.70% vs 18.76%). In addition, there was significantly
less DNA fragmentation in the COCs group than in the control group (18.83 vs 10.83).
Conclusion
Spermatozoa selected using COCs were likely to be effective in terms of sperm quality
and DNA fragmentation.
Keywords
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Article info
Publication history
Published online: October 15, 2019
Accepted:
October 8,
2019
Received in revised form:
September 23,
2019
Received:
May 30,
2019
Identification
Copyright
© 2019 Elsevier B.V. All rights reserved.
