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Effect of sperm selection method by cumulus oophorus complexes and conventional sperm preparation method on sperm quality and DNA fragmentation for assisted reproduction techonology

Published:October 15, 2019DOI:https://doi.org/10.1016/j.ejogrb.2019.10.004

      Abstract

      Objectives

      To compare the effectiveness of sperm selection using cumulus oophorus complexes (COCs) and conventional sperm preparation methods on sperm quality and DNA fragmentation

      Methods

      Normal semen samples under the World Health Organization (WHO)’s 2010 eligibility criteria were collected and processed using conventional sperm preparation methods. The prepared sperm were divided into two groups. Spermatozoa in the study group were selected based on their ability to penetrate a layer of COCs. In the control group, spermatozoa were kept in culture medium under similar conditions. The selected-sperm were evaluated based on sperm quality and DNA fragmentation.

      Results

      Thirty normal semen samples were recruited. Spermatozoa that were able to passthrough the COCs had significantly higher sperm motility parameters than the control group (curvilinear velocity [VCL; 143.5 vs 122.2; P < 0.01], average path velocity [VAP; 83.6 vs 69.3; P < 0.01], straight-line velocity [VSL; 67.95 vs 60.45; P < 0.01]). The percentage of normal spermatozoa morphology in the COCs group was significantly higher than in the control group (21.70% vs 18.76%). In addition, there was significantly less DNA fragmentation in the COCs group than in the control group (18.83 vs 10.83).

      Conclusion

      Spermatozoa selected using COCs were likely to be effective in terms of sperm quality and DNA fragmentation.

      Keywords

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      References

        • Practice Committee of the American Society for Reproductive Medicine
        Diagnostic evaluation of the infertile male: a committee opinion.
        Fertil Steril. 2015; 103: 18-25
        • Palermo G.D.
        • Neri Q.V.
        • Takeuchi T.
        • Rosenwaks Z.
        ICSI: where we have been and where we are going.
        Semin Reprod Med. 2009; 27: 191-201
        • Worrilow K.C.
        • Eid S.
        • Woodhouse D.
        • Perloe M.
        • Smith S.
        • Witmyer J.
        • et al.
        Use of hyaluronan in the selection of sperm for intracytoplasmic sperm injection (ICSI): significant improvement in clinical outcomes--multicenter, double-blinded and randomized controlled trial.
        Hum Reprod Oxf Engl. 2013; 28: 306-314
        • Luna D.
        • Hilario R.
        • Dueñas-Chacón J.
        • Romero R.
        • Zavala P.
        • Villegas L.
        • et al.
        The IMSI procedure improves laboratory and clinical outcomes without compromising the aneuploidy rate when compared to the classical ICSI procedure.
        Clin Med Insights Reprod Health. 2015; 9: 29-37
        • Zhuo L.
        • Kimata K.
        Cumulus oophorus extracellular matrix: its construction and regulation.
        Cell Struct Funct. 2001; 26: 189-196
        • Parmegiani L.
        • Cognigni G.E.
        • Ciampaglia W.
        • Pocognoli P.
        • Marchi F.
        • Filicori M.
        Efficiency of hyaluronic acid (HA) sperm selection.
        J Assist Reprod Genet. 2010; 27: 13-16
        • Huszar G.
        • Ozenci C.C.
        • Cayli S.
        • Zavaczki Z.
        • Hansch E.
        • Vigue L.
        Hyaluronic acid binding by human sperm indicates cellular maturity, viability, and unreacted acrosomal status.
        Fertil Steril. 2003; 79: 1616-1624
        • Zhuo L.
        • Yoneda M.
        • Zhao M.
        • Yingsung W.
        • Yoshida N.
        • Kitagawa Y.
        • et al.
        Defect in SHAP-hyaluronan complex causes severe female infertility. A study by inactivation of the bikunin gene in mice.
        J Biol Chem. 2001; 276: 7693-7696
        • Sousa M.
        • Cremades N.
        • Alves C.
        • Silva J.
        • Barros A.
        Developmental potential of human spermatogenic cells co-cultured with Sertoli cells.
        Hum Reprod Oxf Engl. 2002; 17: 161-172
        • Hong S.J.
        • Chiu P.C.
        • Lee K.F.
        • Tse J.M.Y.
        • Ho P.C.
        • Yeung W.S.B.
        Establishment of a capillary-cumulus model to study the selection of sperm for fertilization by the cumulus oophorus.
        Hum Reprod Oxf Engl. 2004; 19: 1562-1569
        • Franken D.R.
        • Bastiaan H.S.
        Can a cumulus cell complex be used to select spermatozoa for assisted reproduction?.
        Andrologia. 2009; 41: 369-376
        • Hong S.-J.
        • Chiu P.C.-N.
        • Lee K.-F.
        • Tse J.Y.-M.
        • Ho P.-C.
        • Yeung W.S.-B.
        Cumulus cells and their extracellular matrix affect the quality of the spermatozoa penetrating the cumulus mass.
        Fertil Steril. 2009; 92: 971-978
        • Cao X.-W.
        • Lin K.
        • Li C.-Y.
        • Yuan C.-W.
        [A review of WHO Laboratory Manual for the Examination and Processing of Human Semen (5th edition)].
        Zhonghua Nan Ke Xue Natl J Androl. 2011; 17: 1059-1063
        • Wang C.
        • Feng G.
        • Shu J.
        • Zhou H.
        • Zhang B.
        • Chen H.
        • et al.
        Cumulus oophorus complexes favor physiologic selection of spermatozoa for intracytoplasmic sperm injection.
        Fertil Steril. 2018; 109: 823-831
        • Fernández J.L.
        • Muriel L.
        • Goyanes V.
        • Segrelles E.
        • Gosálvez J.
        • Enciso M.
        • et al.
        Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion test.
        Fertil Steril. 2005; 84: 833-842
        • Sullivan R.
        • Duchesne C.
        • Fahmy N.
        • Morin N.
        • Dionne P.
        Protein synthesis and acrosome reaction-inducing activity of human cumulus cells.
        Hum Reprod Oxf Engl. 1990; 5: 830-834
        • Hong S.
        • Tse J.Y.M.
        • Ho P.
        • Yeung W.S.
        Cumulus cells reduce the spermatozoa-zona binding inhibitory activity of human follicular fluid.
        Fertil Steril. 2003; 79: 802-807
        • Parmegiani L.
        • Cognigni G.E.
        • Bernardi S.
        • Troilo E.
        • Ciampaglia W.
        • Filicori M.
        “Physiologic ICSI”: hyaluronic acid (HA) favors selection of spermatozoa without DNA fragmentation and with normal nucleus, resulting in improvement of embryo quality.
        Fertil Steril. 2010; 93: 598-604
        • Fulford G.R.
        • Katz D.F.
        • Powell R.L.
        Swimming of spermatozoa in a linear viscoelastic fluid.
        Biorheology. 1998; 35: 295-309
        • Tesarik J.
        • Kopecny V.
        Assembly of the nucleolar precursor bodies in human male pronuclei is correlated with an early RNA synthetic activity.
        Exp Cell Res. 1990; 191: 153-156
        • Pereda J.
        • Coppo M.
        An electron microscopic study of sperm penetration into the human egg investments.
        Anat Embryol (Berl). 1985; 173: 247-252
        • Van Waart J.
        • Kruger T.F.
        • Lombard C.J.
        • Ombelet W.
        Predictive value of normal sperm morphology in intrauterine insemination (IUI): a structured literature review.
        Hum Reprod Update. 2001; 7: 495-500
        • Yagci A.
        • Murk W.
        • Stronk J.
        • Huszar G.
        Spermatozoa bound to solid state hyaluronic acid show chromatin structure with high DNA chain integrity: an acridine orange fluorescence study.
        J Androl. 2010; 31: 566-572
        • Nasr-Esfahani M.H.
        • Razavi S.
        • Vahdati A.A.
        • Fathi F.
        • Tavalaee M.
        Evaluation of sperm selection procedure based on hyaluronic acid binding ability on ICSI outcome.
        J Assist Reprod Genet. 2008; 25: 197-203
        • Yeung W.S.B.
        • Lee K.-F.
        • Koistinen R.
        • Koistinen H.
        • Seppälä M.
        • Chiu P.C.N.
        Effects of glycodelins on functional competence of spermatozoa.
        J Reprod Immunol. 2009; 83: 26-30