Abstract
Objective
This study investigates the association of C1q gene (rs292001 and rs294183) polymorphisms
in HIV infected and uninfected preeclamptic women of African ancestry.
Materials and methods
The study population consisted of 325 pregnant women of African ancestry grouped into
145 normotensive pregnant women (72 HIV uninfected normotensive, 73 HIV infected normotensive)
and 180 preeclamptic pregnant women (103 HIV uninfected preeclamptics, 77 HIV infected
preeclamptics). Preeclamptic pregnant women were further sub-grouped into 79 early-onset
preeclampsia (EOPE) (40 HIV uninfected EOPE, 39 HIV infected EOPE) and 101 late-onset
preeclampsia (LOPE) (63 HIV uninfected LOPE, 38 HIV infected LOPE). Genotyping of
complement C1q gene polymorphisms (rs292001 and rs294183) was detected using a TaqMan®
SNP Genotyping assay from purified DNA.
Results
No significant differences in allelic and genotype frequencies of rs292001 and rs294183
between preeclamptic and normotensive women were observed. Likewise, there were no
significant differences in allelic and genotype frequencies between HIV infected normotensive
vs HIV infected preeclampsia and HIV uninfected normotensive vs HIV uninfected preeclampsia for both SNPs. However, the odds ratio of preeclamptic
women having the GA genotype was 1:2.
Conclusion
We demonstrate that SNPs of the C1q gene (rs292001 and rs294183) are not associated
with the pathogenesis of PE development in women of African ancestry. The role of C1q rs292001 heterozygous GA is highlighted (with and without HIV infection) may affect
susceptibility to PE development. Notably, this dysregulation may affect C1q translation
and protein output thus influencing the downstream role of the complement system and
functional immunology in HIV infection comorbid with PE.
Keywords
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Article info
Publication history
Published online: January 25, 2023
Accepted:
January 24,
2023
Received:
October 27,
2022
Identification
Copyright
© 2023 Elsevier B.V. All rights reserved.
