Highlights
- •The results highlight the importance of exact evaluation of post-ovarian transplant.
- •The benefit of the CAM culture is tissue viability confirmation after warming.
- •The first sign of successful ovarian transplantation can be observed on day 3.
- •Vitrified tissues showed higher viability and transplantation rate vs slow freezing.
- •Vitrification can be considered as a reliable alternative for slow freezing.
Abstract
Objectives
The first days of post-ovarian transplantation are critical periods, as the ischemic
injury can diminish the success rate. In this study, the first day’s events of ovarian
transplantation in two dimensions of structure and ultrastructure following slow freezing
and vitrification were assessed.
Study design
Ovarian tissues (OTs) from 10 cancerous patients were frozen in two methods of slow
freezing and vitrification. Tissues were transplanted onto the CAM and then retrieved
at 5 and 10 days of culture. Nine groups were assigned as follows; I-III; fresh, 5
and 10 days culture, IV-VI; vitrification, 5 and 10 days culture, and VII-IX; slow
freezing, 5 and 10 days culture. Structural and ultra-structural studies were done
to assess the tissue viability and integrity following CAM transplantation. Image
J software was used to measure the amounts of fibrosis and necrosis.
Results
The first sign of successful transplantation was found on day 3 post-transplantation.
Vitrified tissues showed higher viability and transplantation rate compared to the
slow frozen group (65% vs 57.5%) (p = 0.7). Tissue fibrosis and areas didn’t increase
significantly after cryopreservation using two methods (p > 0.05). The areas of fibrosis
and necrosis and avian vessels increased significantly after 5 and 10 days of culture
(p < 0.05). Large ultra-structural follicular deformities were noticed after 10 days
of CAM transplantation. Better stromal ultrastructure features can be found after
vitrified tissue culture. Also, the CAM transplantation technique had negative effects
on the integrity of follicles, independent of the freezing procedure.
Conclusion
Evaluation of early events of the ovarian post-transplantation is of amount importance,
since the hypoxia during this period may accelerate follicular pool depletion, before
the tissue stability. Vitrification can be considered a reliable alternative for slow
freezing. CAM transplantation is a good technique for confirmation of tissue viability
after warming but damaged the follicle ultrastructure in a short period.
Keywords
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Article info
Publication history
Published online: March 14, 2023
Accepted:
March 13,
2023
Received in revised form:
November 29,
2022
Received:
August 13,
2022
Identification
Copyright
© 2023 Elsevier B.V. All rights reserved.
