Abstract
Objective: The aim of this study was to examine the effect of manganese (Mn) alone and with
the addition of copper (Cu) in the inhibition of osteopenia induced by ovariectomy
(OVX) in rats. Study conditions: Four lots of 100-day-old female Wistar rats were divided into experimental groups
of 15 each. One group received a diet supplemented with 40 mg/kg of Mn per kilogram
of feed (OVX+Mn). The second group received the same diet as the first, but with an
additional 15 mg/kg of copper (OVX+Mn+Cu). The third group of 15 OVX and the fourth
group of 15 Sham-OVX received no supplements. At the conclusion of the 30-day experiment,
the rats were slaughtered and their femurs and fifth lumbar vertebrae were dissected.
Femoral and vertebral length were measured with caliper and bones were weighed on
a precision balance. The bone mineral content (BMC) and bone density (BMD) of the
femur (F-BMC, mg and F-BMD, mg/cm2) and the fifth lumbar vertebra (V-BMC, mg and V-BMD, mg/cm2) were measured separately with dual energy X-ray absorptiometry. Results: The F-BMD, mg/cm2 was lower in the OVX than in the Sham-OVX group (P<0.0001) and in the other two groups receiving mineral supplements (P<0.005 in both). F-BMC, mg was significantly lower in the OVX group than in the other
three (P<0.0001 in all cases). Calculations for V-BMC, mg and V-BMD, mg/cm2 are similar to findings in the femur. Conclusions: These data show that a Mn supplement is an effective inhibitor of loss of bone mass
after OVX, both on the axial and the peripheral levels, although this effect is not
enhanced with the addition of Cu.
Keywords
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Article info
Publication history
Accepted:
August 19,
1999
Received:
June 17,
1999
Identification
Copyright
© 2000 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.
